| | 1 | = PoPLaR: BioMethodology - Improve Cell Survival = |
| | 2 | |
| | 3 | - Reduce the irradiation time |
| | 4 | - Gain an accurate understanding of how long HeLa cells can withstand vertical |
| | 5 | - Current limits are that they can survive 20 minutes but not ~1.5 hours |
| | 6 | - Being aware of the issue |
| | 7 | - Reducing the time between shots from 20s to maybe 1s, definitely to 5s |
| | 8 | - Not irradiating RCF in the same carousel |
| | 9 | - A combination of these two would bring the irradiation time from roughly 16 mins to roughly 4 mins |
| | 10 | - Dealing with potential causes: Temperature, CO2, lack of media |
| | 11 | - Heat the carousel in which the cells are placed |
| | 12 | - Keep the carousel rotating so that the cells are consistently wet |
| | 13 | - Change the cell dish lid so that more media can be added through a second hole |
| | 14 | - Would also help regulate temperature |
| | 15 | - Change the cell line from HeLa to the super-resistant ones used in Marie's lab |
| | 16 | - Requires testing the cells at Birmingham and repeating the experiments |
| | 17 | - Not certain it will work |
| | 18 | - Equipment factors |
| | 19 | - Place a thermostat in the incubator to check that it is not too warm |
| | 20 | - Robbie has said he will build a monitor using a Raspberry Pi |
| | 21 | - Use Marie's lab to allow easier observation |
| | 22 | - Need to inform Marie of when so that she can book in the incubator |
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