Changes between Version 3 and Version 4 of Research/LhARA/RadiationBiology/BMthdlgy/ImprvCllSrvvl


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Timestamp:
Apr 16, 2026, 5:44:39 PM (7 weeks ago)
Author:
ccd24
Comment:

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  • Research/LhARA/RadiationBiology/BMthdlgy/ImprvCllSrvvl

    v3 v4  
    22
    33- Reduce the irradiation time
    4  1) Gain an accurate understanding of how long HeLa cells can withstand vertical
     4 1. Gain an accurate understanding of how long HeLa cells can withstand vertical
    55  - Current limits are that they can survive 20 minutes but not ~1.5 hours
    6  2) Being aware of the issue
    7  3) Reducing the time between shots from 20s to maybe 1s, definitely to 5s
    8  4) Not irradiating RCF in the same carousel
     6 2. Being aware of the issue
     7 3. Reducing the time between shots from 20s to maybe 1s, definitely to 5s
     8 4. Not irradiating RCF in the same carousel
    99  - A combination of these two would bring the irradiation time from roughly 16 mins to roughly 4 mins
    1010- Dealing with potential causes: Temperature, CO2, lack of media, weak cell line
    11  5) Heat the carousel in which the cells are placed
     11 5. Heat the carousel in which the cells are placed
    1212  - Can this be done by placing the carousel in the incubator as well?
    13  6) Keep the carousel rotating so that the cells are consistently wet
    14  7) Change the cell dish lid so that more media can be added through a second hole
     13 6. Keep the carousel rotating so that the cells are consistently wet
     14 7. Change the cell dish lid so that more media can be added through a second hole
    1515  - Would also help regulate temperature
    16  8) Change the cell line from HeLa to the super-resistant ones used in Marie's lab
     16 8. Change the cell line from HeLa to the super-resistant ones used in Marie's lab
    1717  - Requires testing the cells at Birmingham and repeating the experiments
    1818  - Not certain it will work
    1919- Equipment factors
    20  9) Place a thermostat in the incubator to check that it is not too warm
     20 9. Place a thermostat in the incubator to check that it is not too warm
    2121  - Robbie has said he will build a monitor using a Raspberry Pi
    22  10) Use Marie's lab
     22 10. Use Marie's lab
    2323  - Need to inform Marie of when so that she can book in the incubator
    2424  - Can do x-ray comparison here