= PoPLaR: BioMethodology - Improve Cell Survival =

- Reduce the irradiation time
 - Gain an accurate understanding of how long HeLa cells can withstand vertical
  - Current limits are that they can survive 20 minutes but not ~1.5 hours
 - Being aware of the issue
 - Reducing the time between shots from 20s to maybe 1s, definitely to 5s
 - Not irradiating RCF in the same carousel
  - A combination of these two would bring the irradiation time from roughly 16 mins to roughly 4 mins
- Dealing with potential causes: Temperature, CO2, lack of media, weak cell line
 - Heat the carousel in which the cells are placed
 - Keep the carousel rotating so that the cells are consistently wet
 - Change the cell dish lid so that more media can be added through a second hole
  - Would also help regulate temperature
 - Change the cell line from HeLa to the super-resistant ones used in Marie's lab
  - Requires testing the cells at Birmingham and repeating the experiments
  - Not certain it will work
- Equipment factors
 - Place a thermostat in the incubator to check that it is not too warm
  - Robbie has said he will build a monitor using a Raspberry Pi
 - Use Marie's lab to allow easier observation
  - Need to inform Marie of when so that she can book in the incubator




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