PoPLaR: BioMethodology - Improve Cell Survival

• Reduce the irradiation time
  • Gain an accurate understanding of how long HeLa cells can withstand vertical
    • Current limits are that they can survive 20 minutes but not ~1.5 hours
  • Being aware of the issue
  • Reducing the time between shots from 20s to maybe 1s, definitely to 5s
  • Not irradiating RCF in the same carousel
    • A combination of these two would bring the irradiation time from roughly 16 mins to roughly 4 mins
• Dealing with potential causes: Temperature, CO2, lack of media, weak cell line
  • Heat the carousel in which the cells are placed
  • Keep the carousel rotating so that the cells are consistently wet
  • Change the cell dish lid so that more media can be added through a second hole
    • Would also help regulate temperature
  • Change the cell line from HeLa to the super-resistant ones used in Marie's lab
    • Requires testing the cells at Birmingham and repeating the experiments
    • Not certain it will work
• Equipment factors
  • Place a thermostat in the incubator to check that it is not too warm
    • Robbie has said he will build a monitor using a Raspberry Pi
  • Use Marie's lab to allow easier observation
    • Need to inform Marie of when so that she can book in the incubator

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