= LhARA radiobiology; meeting: 14Jul25; 14:00 BST =

**Present:** CW, RW, CP, EM, JMcG, AFR, CD, KL, DO

== Notes ==

1. //[wiki:Research/LhARA/RadiationBiology/Meetings/2025-06-26 Minutes and actions]: //

  - JP: Talk to Fisher and get a plan of action for the order and delivery of consumables to Strathclyde
   - **STANDS**
  - CD/AFR: Complete verification of RCF stack
   - **STANDS**

2. //Strathclyde Updates: // RW, CW \\
- Laser damage and beamtime:
 - Damage also includes a vacuum pipe that needs fixing, defines a new schedule
 - New Schedule:
  - Week 1: Get back to where we were a month ago with correct laser delivery to the target.
  - Week 2: Re-establish and troubleshoot proton generation.
  - Week 3: Work with the quads and some diagnostic testing in the PoPLaR configuration. 
   - Opportunity for a full bio walkthrough.
   - Double check the autoclave waste 
   - Highly dependent on week 1 and week 2

 - Bio-safety cabinets:
  - Recalibration still required and unsure of schedule

 - RCF stack fiduciary:
  - Smaller stack proving to be tricky
  - Larger stack can be used as a viable back-up
  - Ken may be able to encourage CLF to help with smaller stack 
  - Aligned with a cross-hair on carousel and a laser along the beam axis

6. //Bioprep: // EM/MH \\
 - IF needs staining from the last experiments (14 vs 28 MeV)
 - Issue with Mylar remains - colony survival too variable to be reliable 
 - Tested post-plating FaDu cells from Mylar following 28 MeV protons (0-4Gy) with reliable and consistent colony formation 
 - To do - Rerun cyclotron test experiments comparing 14 and 28 MeV with post-plating method for both HeLa and FaDu - date TBD 
 - To do - stain, image and analyse IF on Mylar 

7. DoNM
 - 22Jul25 14:00 **BST**

8. AoB \\
 - Present work to LhARA fortnightly tomorrow 
  - CD: Updated SCAPA schedule
  - EM: Updates from latest experiment
  - CD: LMU work


=== Summary of actions ===
 **EM:** Comparison of 14 and 28 MeV with post-plating method. Analyse IF on Mylar
 **CW:** Bio-cabinets need recalibration




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