Changes between Version 4 and Version 5 of Research/LhARA/RadiationBiology/Meetings/2025-11-13


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Timestamp:
Nov 13, 2025, 3:47:05 PM (5 weeks ago)
Author:
ccd24
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  • Research/LhARA/RadiationBiology/Meetings/2025-11-13

    v4 v5  
    771. Minutes and actions:
    88- **CW:** Contact Marie Boyd
    9  - **Done** Waiting
     9 - **Done** Waiting. Probably worth waiting till we irradiate cells.
    1010
    11112. Strathclyde: BT, ED, RW, CD, TP, EM \\
    1212- RW: Update on laser
     13 - Front quad is quite shiny so placed something in front to reduce reflection.
     14 - Changed around 5 separate things and now (fingers crossed) do not see the burning...
     15 - Ran 20 shots on Tues and Weds and saw no burning
     16 - Does mean not sure what caused it and could be any of the 5 things, including front quad being reflective...
     17
     18- CD: Recap of experimental and simulated results
     19 - [raw-attachment: Slides]
     20 - Nick pointed out there is still a flaw in the comparison based on the number of particles reaching the final dish
     21 - Simulations producing similar shapes to experimental results
     22 - Based on Highland equation for multiple scattering angle (from Geant4 documentation) predicted the different scattering effects
     23 - Mark has offered 2.5um Gold if we wanted to try that?
     24 - Cu 10um at furthest distance from second quad seems like a sensible option
     25 - If not uniform enough can move it closer
     26 - If we move it so the copper is 300mm from second quad then sims predict the dose will drop by 45% and the CV goes down by 6%
     27 - Robbie has a spare drive that we could use to play with this without pumping down the vacuum chamber each time
     28
    1329- RCF update: Plan to recalibrate
    1430 - EM update on Birmingham calibration
    15  - KL/CD update on getting calibrated film cut
    16 - CD: Recap of experimental and simulated results
    17  - Discuss best route forward
    18 - CD: Discussion of the best metric for uniformity
     31  - Calibration Done
     32  - 2023 batch is much preferred due to the framing we see around the edge of the darker batch
     33  - If we use the darker batch in the future cut only for the centre to avoid the framing
     34  - Calibration was also complete for some of the framed parts of the film to get some values for previously scanned work
     35  - Three sheets of the 2023 batch with Emma
     36  - I think there is more in SCAPA - Ask Ewan
     37 - KL/CD update on 2023 getting cut to 1cmx1cm:
     38  - Hopefully done by tomorrow
     39 - For future use best to buy a new batch and complete a calibration just before irradiating
     40 - Scanner Best Practices:
     41  - Place black cardboard around the RCF on the scanner to fix its position
     42  - Run 5-10 scans before scanning RCF to allow the scanner lamp to warm up
     43 - RCF Dose Calculation:
     44  -[raw-attachment: Slides]
     45  - Key Conclusions:
     46   - Scanner Best Practices:
     47    - Place black cardboard around the RCF on the scanner to fix its position
     48    - Run 5-10 scans before scanning RCF to allow the scanner lamp to warm up
     49   - An x by x median filter kernel should be applied to the dose map before calculating the flatness. Tony uses x=5.
     50   - A three channel correction could provide a more accurate dose than using one channel
     51   - Variability pixel to pixel? - Comes from the scanner resolution and is not indicative of low stats hitting each cell
     52    - 1 Gy means around 70 particles traversing the nucleus
    1953
    20543. BioPrep EM, JP: \\
    2155- EM: Dry run outcome
     56 - Staining to be completed on Monday
    2257- CW: Marie Boyd update
     58 - Wait till we have some cells irradiated
     59- Emma has to be in Oxford on Friday so cells need to be irradiated before then
     60- Possibility of extra days due to laser downtime? - Chat more about this on Monday
    2361
    24624. Simulation CD, DA, AFr: \\
    25 - Majority covered in the Strathclyde update
     63- No update other than sims included above
    2664
    27655. Beam Diagnostics KL, CD: \\
    2866- Real-time beam diagnostic update
    2967 - Lanex tested in the beamline appears to work
    30  - Lanex behind the cell dish?
     68 - Lanex behind the cell dish
    3169
    32706. DoNM
     
    3472
    35737. AoB
    36 - Josie viva
     74- Josie viva - Completed it mate
    3775----