= LhARA radiobiology; meeting: 17Dec25; 12:00 BST = **ZOOM:** https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1 Attended: CD, PH, AFr, MB, RA, KL, EM, HO, JP, MH == Meeting Notes == 1. Minutes and actions: - **MB/EM**: Evaluate the cells - **STANDS** - **CD, AFr**: Complete analysis of RCF with Error Calculation - **STANDS** (Waiting for Orientation Scans) - **EM/ED/LJ**: Send calibration films to SCAPA and scan them in both orientations - **STANDS** - **AFr, DA**: Write out RCF procedure document - **STANDS** - **EM, JMcG**: Write up summary of PoPLaR Phase 2, including technical summary of cell irradiation procedure - **STANDS** - **RW, CD**: Study correlation between laser diagnostics and mean dose - **STANDS** - **CD, TP**: Evaluate LET in the cells with the RCF in front - **STANDS** - **CD**: Compare dose profile consistency - **DONE** 2. SCAPA RW,ED: No Update 3. Bio EM,JP,MB: - See cells in FaDu from all days and the higher seeding densities. - Seeing a clear dose response. - Still looks like the radiation has been too high. - No cells in HeLa. - Seem to be more sensitive. - Emma mentioned that people working with them in Birmingham at the same time as phase 2 were struggling to get results with them. So could be the cell line? However, since Emma has got back she has done more work with them and they seem fine? - Possibly worth moving to a different cell line in the future? - Cells back with Mark and he will organise their delivery to Birmingham. (Most likely after Christmas) - Results hopefully can be produced by end of Jan - Marie is keen to stay connected. She is putting together a grant about different radiation types so this work is all useful. - Emma to share the counting software she uses with Marie - Still uncertain about the uniformity. - Comet analysis should help with this - But cells were on ice for a while before being forzen so may have repaired. - Calvin to send Emma min and max dose 4. Beamline Diagnostic, KL, TP, CD, PH: - RCF Update - RCF not sent up yet, so will send in Jan - KL, PH on Sparse Sci-Fi - [raw-attachment:LhARA_17Dec25.pdf Slides] - Peter has a simulation of the fibres used (50mm long fibre polysterene core and PMMA cladding as per BCF50) - If using a remote camera recieve order of 1/1000 power from scintillation as if using a nearby screen. - Key questions: 1) What is the typical light produced in a sparse fibre per laser pulse - How many protons per shot pass through each fibre - 20-30 is fine but 1 or 2 is too few - Can be done in sim but requires a better understanding of the source distribution 2) Dimensions of the vacuum chamber - Instead of remote could also looking at capturing the light into a larger fibre to collect more light - Phosphor screen requires face-on viewing so possibly some practical difficulties - Peter also happy to simulate this - Using the discarded beam (Photodiode). - [raw-attachment:Beam_Consistency.pptx Slides] - Beam consistency shows a Pearson Correlation Coefficient of minimum 0.83. - Predicting the average pixel count inside the cell dish from the beam outside the cell dish provides a Pearson correlation coefficient of 0.991, and can predict with a 6% error. - Better analysis to be done with dose once RCF properly calibrated - Delaminated RCF - Mark Hill has some and will take it to Birmingham tomorrow. - Will need to do a calibration of this before we start a new run - Least popular option 5. Lessons Learnt and Aims for Timeline, CD: - [raw-attachment:FutureAims.pptx Slides] - Critical issues are: - Beam uniformity - Shot-to-shot variation - Control Survival - Plan for next run (minimum requirements): - Repeat of phase 2 - Move the scatterer forward to improve beam uniformity - Have an in-beam diagnostic - Minimum option is delaminated RCF - Requires calibration and full description of RCF errors - Hopefully test a sparse sci-fi - Reduce the time the cells are in carousel - Reduce time between shots - Make people aware of the importance of this - Use SCAPA as destination for immediate cell work - Require an inverted microscope - At the end of the week move the cells to Marie's lab for better control - Discussion needed on the benefits of doing this run? - Longer-term - Remove scatterer and introduce more quads - Introduce a dipole chicane - Use an X-ray control - Introduce a (pseudo-)beampipe - Look at ELIMED option - Permanent SCAPA beamline? 6. In-Person Meeting - Date: February (9th, 10th, 24th, 25th, 26, 27th are currently the best options) - Place: Strathclyde will be the default. Second choice would be Birmingham - Need to discuss further - lettuceMeet: https://lettucemeet.com/l/lWwgW 7. DoNM - 8th Jan (Only if necessary to sort out deliveries etc) Emma to host 8. AoB - Ken keen for support for Royal Society Exhibition - Merry Christmas!!! === Summary of actions required === - **ALL**: FILL OUT LETTUCEMEET - **ALL**: Decide location for in-person meeting Phase 2 Analysis - **EM, MB**: Organise transport of cells to Birmingham for evaluation - **EM**: Evaluate the cell results - **CD, AFr**: Complete analysis of RCF with errors - **EM/ED/LJ**: Send calibration films to SCAPA and scan them in both orientations - **EM, JMcG**: Write up summary of PoPLaR Phase 2, including technical summary of cell irradiation procedure - **EM**: Share the counting software she uses with Marie - **MB**: Comet analysis - **CD**: Tell Emma the min and max dose seen on the cell dish Beam Diagnostics - **AFr, DA**: Write out RCF procedure document - **Unassigned**: Find all the errors associated with RCF - **RW, CD**: Study correlation between laser diagnostics and mean dose - **CD, TP**: Evaluate LET in the cells with the RCF in front - **RW, ED**: Tell Peter the dimensions of the vacuum chamber - **Unassigned (CD)**: Use sim to answer how many protons per shot in each fibre Improve Bio results - **EM, JP**: Discuss cell line to use next time? - **Unassigned**: Obtain an inverted microscope Long-term - **CD, JMcG**: Investigate how to achieve uniformity without a scatterer in place ----