= LhARA radiobiology; meeting: 05Feb26; 14:00 BST =

**ZOOM:** https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1 

Attended: KL, RW, CW, RA, PH, MB, AFr, MH, EM, CD

== Meeting Notes == 

1. Minutes and actions completed:
 - **AFr, DA**: Write out RCF procedure document
  - **DONE**
 - **CD**: Use sim to answer how many protons per shot in each fibre
  - **DONE** 

2. In-Person Meeting
- Date: DECIDED 24th Feb at Birmingham
- Discussion on location
 - Confirmed Birmingham. Jason and Emma to book a room for max 20 people.
 - Invite LMU to join online
 - Invite Liverpool to share their work on air scintillation
 - Invite Chris Armstrong to join for instrumentation section
- Draft Agenda: https://indico.stfc.ac.uk/event/1834/ 
 - Make clear to include timetables for SCAPA and ELIMED (and LMU)
 - Make clear a section on improved survival rates of cells

3. SCAPA RW,ED:
- General Update
 - Laser fixed
 - Running a DSTL till mid-march, going to be around April before access again
 - Josie asked for Dipoles from IoM charity. Waiting outcome

4. Bio EM,JP,MB:
- Actions Checklist
 - **MB/EM**: Send and evaluate the cells
  - Delivered
 - **EM, JMcG**: Write up summary of PoPLaR Phase 2, including technical summary of cell irradiation procedure
 - **EM**: Share the counting software she uses with Marie
  - **Done**
  - Oxford Optronix, Gelcount
 - **MB**: Comet analysis
- General Updates
 - Cells have been delivered to Birmingham
  - Looks like nothing on HeLa. FaDu looks more promising
  - Caution required due to dose variation
 - Before next run inform Marie the dates so she can book out the incubator to be used
 - Ideas to improve cell survival rates
  - Swap out HeLa with Glioma cell line (EBW). More robust.
   - Would need to send some to Birmingham
   - Emma to email Rosh about when to take them out the freezer
  - Dry runs indicate that if the cells are only out for 20/25mins then good survival
   - Remove the RCF after irradiation reduces time drastically
  - Cells could be too cold
   - Heating the whole holder could provide enough heat to keep the cells warm for longer
  - Cells could be hypoxic
   - Pre-gas the cells?
  - Keep the holder rotating so that the cells stay wet during irradiation

5. Beamline Diagnostic, KL, TP, CD, PH:
- Actions Checklist
 - **EM/ED/LJ**: Send calibration films to SCAPA and scan them in both orientations
  - Delivered
 - **AFr, DA**: Write out RCF procedure document
  - **DONE**
 - **CD, TP**: Evaluate LET in the cells with the RCF in front
 - **CD**: Use sim to answer how many protons per shot in each fibre
  - **DONE**
- Protons per Fibre - CD
 - [raw-attachment:ProtonsPerFibre.pptx Slides]
 - Estimating that the beam delivers 0.2mJ per shot per fibre, which works to 200W
 - Oversaturation problem?
- Overview of RCF document
 - [raw-attachment:"RCF Scanning Protocols for LhARA (2026).pdf" Doc]
- General Updates
 - CD, RW and AFr to discuss the best way to scan RCF


6. DoNM 
- 19th Feb 14:00

7. AoB 
- Prepare a summary for LhARA fortnightly on the day of the meeting

=== Summary of actions required ===

Phase 2 Analysis
- **EM**: Evaluate the cell results
- **CD, AFr**: Complete analysis of RCF with errors
- **EM/ED/LJ**: Send calibration films to SCAPA and scan them in both orientations
 - **CD, RW and AFr**: Explain scanning procedure
- **EM, JMcG**: Write up summary of PoPLaR Phase 2, including technical summary of cell irradiation procedure
- **MB**: Comet analysis
- **CD**: Invite Liverpool, Chris Armstrong and LMU to join the in-person meeting

Beam Diagnostics
- **Unassigned**: Find all the errors associated with RCF
- **RW, CD**: Study correlation between laser diagnostics and mean dose
- **CD, TP**: Evaluate LET in the cells with the RCF in front

Improve Bio results
- **EM**: Inform Roshine when to defrost Glioma cell line
- **Unassigned**: Obtain an inverted microscope

Long-term
- **CD, JMcG**: Investigate how to achieve uniformity without a scatterer in place
- **KL, CW**: Cost 4 or 6 quad, and chicane systems

----