| 47 | | - Solutions to the three main problems: |
| 48 | | - Move the scatterer further up the beamline (closer to the laser-target). |
| 49 | | - Use a real-time diagnostic |
| 50 | | - Investigate how long the cells survive and the cause of death |
| 51 | | - Additions: |
| 52 | | - Collect more biology results? |
| 53 | | - When?: |
| 54 | | - Robbie mentioned February |
| 55 | | - Need to be ready to latch onto any downtime of other experiments |
| 56 | | - More information: https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?tab=t.0 |
| 57 | | - Steps required: Below and at https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar |
| | 47 | - Solutions to the three main problems: |
| | 48 | - Move the scatterer further up the beamline (closer to the laser-target). |
| | 49 | - Use a real-time diagnostic |
| | 50 | - Investigate how long the cells survive and the cause of death |
| | 51 | - Additions: |
| | 52 | - Collect more biology results? |
| | 53 | - When?: |
| | 54 | - Confirmation: earliest possible beamtime is February, as this is when the tape drive is being put back in. |
| | 55 | - More information: https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?tab=t.0 |
| | 56 | - Steps required: Below and at https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar |
| 60 | | - Options: |
| 61 | | - Sparse scintillating fibre arrays in the vacuum chamber |
| 62 | | - Scintillating fibre around the cell dish |
| 63 | | - Lanex around the cell dish |
| 64 | | - Integrated Current Transformers (ICTs) |
| 65 | | - Laser Diagnostics (Backscatter etc) |
| 66 | | - Replace the scatterer with a scintillating sheet |
| 67 | | - (RCF around the cell dish (if desperate)) |
| 68 | | - Testing method: |
| 69 | | - Calibrate with RCF? |
| 70 | | - No calibration. Use lots of cell dishes and match the distribution of variation in diagnostic output to the "known" distribution of the shot-to-shot variation |
| 71 | | - LET Detector |
| | 59 | - Options: |
| | 60 | - Sparse scintillating fibre arrays in the vacuum chamber |
| | 61 | - Scintillating fibre around the cell dish |
| | 62 | - Lanex around the cell dish |
| | 63 | - Integrated Current Transformers (ICTs) |
| | 64 | - Laser Diagnostics (Backscatter etc) |
| | 65 | - Replace the scatterer with a scintillating sheet |
| | 66 | - (RCF around the cell dish (if desperate)) |
| | 67 | - Discussion: |
| | 68 | - Other diagnostics: |
| | 69 | - Should include Liverpool's fluorescence. |
| | 70 | - **Colin to contact Narender about implementation** |
| | 71 | - Suggestion to use the fast electrons as a diagnostic |
| | 72 | - Nick also has his ToF for future times when we have money |
| | 73 | - Scifi: |
| | 74 | - Test clear fibre in a beam. If it scintillates then can use it in the scifi array. If it doesn't can use it to transport the light out the vacuum chamber from the scifi array |
| | 75 | - Robbie offered to test at ELI. He leaves on the 16th August. |
| | 76 | - **Ken and Calvin to send to SCAPA before then (Contact Ewan or Colin since Robbie is USA)** |
| | 77 | - If no time can always test at Swansea or Birmingham |
| | 78 | - Lanex: |
| | 79 | - Fear of Lanex quenching at high rep rates but this is more of a concern near the laser target rather than at the cell dish |
| | 80 | - Testing Method: |
| | 81 | - Agreement that we should calibrate with RCF before hand |
| | 82 | - However, also calibrate a batch of RCF that is scanned after a shorter time period (not 24/48 hrs) |
| | 83 | - Use this for continuous calibration of whichever diagnostic is used during cell irradiations |
| 74 | | - Spatial Variation: Is a CV of 8% acceptable? |
| 75 | | - Cell Control Survival: |
| 76 | | - Need to test how long cells can remain vertical |
| 77 | | - Can reduce the time taken to conduct the irradiations (and therefore the time the cells are vertical for) |
| 78 | | - Monitor the temperature of the thermostat |
| 79 | | - Better instrumentation: |
| 80 | | - Inverted microscope |
| 81 | | - Multi-chamber haemocytometer |
| 82 | | - More results (as well as clonogenics): |
| 83 | | - Comet analyses |
| 84 | | - X-ray comparison on-site |
| | 86 | - Spatial Variation: Is a CV of 8% acceptable? |
| | 87 | - Diaza has read a lot of literature that says 5% should be the maximum |
| | 88 | - Need to figure out how to improve the beamline (Move PMQs) |
| | 89 | - Cell Control Survival: |
| | 90 | - Need to test how long cells can remain vertical |
| | 91 | - **Josie to plan a test** |
| | 92 | - Investigate: |
| | 93 | - Vertical or time out of incubator |
| | 94 | - Emma/Jason's HeLa cell line |
| | 95 | - Marie's cell line |
| | 96 | - Also look at evolving a group of cells that can survive the Mylar |
| | 97 | - Can reduce the time taken to conduct the irradiations (and therefore the time the cells are vertical for) |
| | 98 | - Monitor the temperature of the thermostat |
| | 99 | - Better instrumentation: |
| | 100 | - Emma and Jason looking at a glass ring setup so that the cells could remain submerged in media |
| | 101 | - Potential to leak... |
| | 102 | - Investigate thicker Mylar as less likely to poke through with pipette |
| | 103 | - Can get up to 1mm Mylar |
| | 104 | - There is an inverted microscope for £250: Openflex 3D printable one with raspberry pi. Could automate counting? |
| | 105 | - More results (as well as clonogenics): |
| | 106 | - Comet analyses |
| | 107 | - Agreed to do |
| | 108 | - X-ray comparison on-site |
| | 109 | - IF: |
| | 110 | - Very useful for SFRT |
| | 111 | - Also, provides a potential internal dosimeter: 30 dots = 1Gy |
| | 112 | - Could store two samples and use one as the dosimeter |
| | 113 | - Would have to be consistent with when the cells are fixed and stained |
| | 114 | - Need to test further: **Josie to look into this. Discuss with Emma** |
| | 115 | - Concern is the labour to check at every timepoint |
