| 7 | | 1. General Discussion |
| 8 | | - Robbie mentioned that beamtime might be more likely in February. |
| 9 | | - Colin believes we might be able to get some time in Autumn if we can time it correctly with other beamtime. |
| 10 | | - There is work where the SCAPA team are using AI and looking at the variability of the laser. |
| 11 | | - Colin to discuss with Robbie how to build the setup with another experiment |
| | 7 | 1. Overview of Current Plan |
| | 8 | - Three main problems are: |
| | 9 | - Spatial variation |
| | 10 | - Shot-to-shot variation |
| | 11 | - Survival of the control cells |
| | 12 | - Solutions: |
| | 13 | - Move the scatterer further up the beamline (closer to the laser-target). |
| | 14 | - Use a real-time diagnostic |
| | 15 | - Investigate how long the cells survive and the cause of death |
| | 16 | - Additions: |
| | 17 | - Collect more biology results? |
| | 18 | - When?: |
| | 19 | - Robbie mentioned February |
| | 20 | - Need to be ready to latch onto any downtime of other experiments |
| | 21 | - More information: https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?tab=t.0 |
| | 22 | - Steps required: Below and at https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar |
| 14 | | - General |
| 15 | | - Colin suggested filling the carousel and running 1 shot then repeating with 2 shots etc |
| 16 | | - Each cell dish would have Lanex around it or something for real-time measurement |
| 17 | | - Then we could skip the calibration stage by comparing the variation in the Lanex signal with the known variation in dose |
| 18 | | - Problems involve the number of cells needing to be collected and the time that would take |
| 19 | | - Needs discussion with bio team |
| 20 | | - SciFi |
| 21 | | - Need to actually test whether the transporting fibre will scintillate |
| 22 | | - Operate on the assumption it does not |
| 23 | | - If it does Peter has ordered hollow-fibre |
| 24 | | - Might be able to use a standrad fibre-optic connector |
| 25 | | - ICT |
| 26 | | - Colin has ICTs |
| 27 | | - 3-5ns rise time, so should see the short pulses but on the edge of visible and may be blurred out |
| 28 | | - 0.1V in signal per Amp in beam current |
| 29 | | - Beam would also need to be roughly symmetric, so has to be after the scatterer |
| 30 | | - There is also a smaller, more sensitive ICT we could use |
| 31 | | - Also possibilities with 3D printing |
| | 25 | - Options: |
| | 26 | - Sparse scintillating fibre arrays in the vacuum chamber |
| | 27 | - Scintillating fibre around the cell dish |
| | 28 | - Lanex around the cell dish |
| | 29 | - Integrated Current Transformers (ICTs) |
| | 30 | - Laser Diagnostics (Backscatter etc) |
| | 31 | - Replace the scatterer with a scintillating sheet |
| | 32 | - (RCF around the cell dish (if desperate)) |
| | 33 | - Testing style: |
| | 34 | - Calibrate with RCF? |
| | 35 | - No calibration. Use lots of cell dishes and match the distribution of variation in diagnostic output to the "known" distribution of the shot-to-shot variation |
| | 36 | - LET Detector |
| 33 | | 3. Overview of Trello |
| 34 | | - Reminder plan is laid out in the google doc and can be changed |
| 35 | | - Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar |
| 36 | | - Planning next SCAPA Document: https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?usp=sharing |
| | 38 | 3. BioPrep |
| | 39 | - Spatial Variation: Is a CV of 8% acceptable? |
| | 40 | - Cell Control Survival: |
| | 41 | - Need to test how long cells can remain vertical |
| | 42 | - Can reduce the time taken to conduct the irradiations (and therefore the time the cells are vertical for) |
| | 43 | - Monitor the temperature of the thermostat |
| | 44 | - Better instrumentation: |
| | 45 | - Inverted microscope |
| | 46 | - Multi-chamber haemocytometer |
| | 47 | - More results: |
| | 48 | - Standard is clonogenics |
| | 49 | - Marie's Lab can do comet analyses |
| | 50 | - They could also do an X-ray comparison on-site |
