= LhARA Radiobiology Meeting: 20Aug26; 14:00 BST = **ZOOM:** https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1 - Planning Document: https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?tab=t.0 - Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar == Meeting Notes == 1. Beamline Simulation - Josie: Characterisation of ELI and SCAPA sources [raw-attachment:eli_scapa_poplar_update_20_08_26.pptx Slides] - Using the LLO simulation and changing the electron temperature in the model to match the energy spectra for the ELI and SCAPA beamlines - SCAPA fitted with 5.5MeV electron temperature - ELI fitted with 0.25MeV electron temperature - Will chat with Ewan to discuss further - Need information on the source divergence - Josie: Simulations of a dipole at SCAPA - Have funding for dipoles - First introduction of dipoles indicates a 90% beam loss - Need a more accurate measurement of the space to work in - Can test at Birmingham - Josie to talk to Darsebury to get more information on sensible dipole parameters - Next beamtime will probably not include this dipole arrangement 3. BioPrep - Alex's plan: [raw-attachment:AlexMTranswells.pptx Slides] - Use transwell with 10um membrane - Use absorbent paper on the side of transwell with media - Don't need cells to be in media - Concern that as the cells/paper dries the thcikness of media will change and the cell will receive a different dose? - No cell lid and no media means we can place RCF behind the cells - With the cells being vertical little concern over contamination - Could cover all but one of the holes on the carousel with a Perspex cover - This will keep the moisture in the dish - Uncover just to irradiate and then rotate back under - This also works for a vertical beam so sterilisation is not an issue - Maybe add a second plastic screen above the beamline just in case - Can always use antibacterial media - Have 6, 12, and 24 well plates so we can cluster the cells that all see the same dose ie reduces the spatial variation. - Also could flash-freeze the media and do analysis on that (bystander) - Whether electrical impedance to check the cell layer is a monolayer and other techniques - Need to dry run and understand cell survival - Colin to take Alex on a tour of SCAPA (Aiming for next week) - Need an understanding of the publishable minimum - Roshine and Marie to draft a plan and to share with Emma and Jason (and Josie) - Incubator downstairs in SCAPA? - Consider the CO2 - Minimum repeats is 3 - Minimum cell lines is 2 - Which cell lines - Emma and Jason prefer their HeLa and FaDu since already done Birmingham runs with them - Think about using the hardier cell lines from Glasgow as well 3. Instrumentation - Fast Electrons - Giuseppe and Richard H. looking at this and need to find the apparatus with the right parameters for our system - Ken to visit on October - RCF - Mark has found some EBT4 delaminated: https://www.ashland.com/industries/medical/radiotherapy-films/ebt4 - Tony to inquire to see if he can get hold of some - Alex also suggests casting the RCF into a thinner sheet by using an organic solvent and casting it onto a silicon sheet. - Air Scintillation - Colin/Narendar: Planning to meet to discuss the feasibility of the implementation at SCAPA and the timeframe 4. SCAPA - Updates: None (On experiment) 5. Review Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar 6. DoNM - 03/09/26 7. AoB === Summary of actions required === In-Beam Diagnostics - **Unassigned**: Acquire a glass sheet - **Unassigned**: Get a new RCF batch and calibrate - **TP**: Testing delaminating EBT3 - **PH**: Obtain transparent fibre or an alternative method for light transport - **PH**: Obtain a lens for hollow fibre tests - **CD**: Simulate energy deposited in transport fibres - **KL, CD, JMcG, PH**: Design a system to make full scifi prototypes - **KL, CD, JMcG, PH**: Make a full scifi prototype - **KL, CD**: Send clear fibre to SCAPA before 16th August (Contact Ewan or Colin since Robbie is USA) - **KL, CD, JMcG, PH**: Discuss visit to Birmingham to test SciFi - **RW, CD**: Study the correlation between laser diagnostics and mean dose - **Unassigned**: Investigate how a phosphor sheet could be incorporated into the design of the cell dish - **CW**: Investigate the feasibility of ICTs for our beamline - **MH**: Investigate LET detector - **CW**: Ask Narender about air fluorescence implementation - **JMcG**: Investigate using IF as a dosimeter - **GS, RH**: Investigate fast electron diagnostic - **TP**: Investigating commerical delaminated EBT4 Bio Next Steps - **EM, CD, JMcG**: Write up technical summary of Phase 2 - **EM**: Write up a biology plan for a 2 PMQ setup - **Unassigned**: Obtain an inverted microscope - **Unassigned**: Obtain multi-chamber haemocytometers (Might be able to use Birmingham's) - **Unassigned**: Obtain a thermostat for the incubator - **EM**: Run controls to examine how long the cells can survive vertically - **JMcG**: Plan a test of vertical cell survival using Emma/Jason's HeLa cell line and Marie's cell line - **JMcG**: Produce a HeLa colony that can survive on Mylar - **CW, AM**: Show Alex around SCAPA Beamline Modelling - **Unassigned**: Investigate how to improve the beamline beyond CV=8% - **CD**: Examine energy spectra variation - **JMcG, ED**: Discuss spectra fits and SCAPA source divergence - **JMcG**: Talk to Darsebury to get more information on sensible dipole parameters Alternate Beamlines - **JMcG**: Simulate BELLA - **JMcG**: Simulate ELI ----