= LhARA Radiobiology Meeting: 03Sep26; 14:00 BST = **ZOOM:** https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1 - Planning Document: https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?tab=t.0 - Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar == Meeting Agenda == 1. Alternate Beamlines - ELI proposal feedback - Got rejected for several reasons but most are things we can deal with and the evaluators were generally positive and encouraged resubmission - Next proposals for ELI have a deadline in October - Beamtime next year - Ken checking whether ELIMED is part of that - Calvin to make a planning document so we can list the challenges and fill out how we will deal with them - Discuss with ELI instrumentation team to make acceptance as likely as possible - Discussion on beam energy - Marie and Rosh have done a gap analysis and found one looking at <10MeV protons - Fear that focussing on lower energy will not appeal to ELI since they have the technology to reach 60MeV - ELI also appears to have lower beam quality as you go to lower energy - Jason has comparison at Clatterbridge for 60MeV and 28MeV - Could suggest higher energies but also no clear indication that lower energy was a reason we got rejected since we did not mention beam energy in the original submission 2. BioPrep - Ken and Josie did a review - Ken showed a figure showing cell death and very little difference between laser-driven and conventional but also multi-pulse and single shots - Aim should be to improve on this - Rosh and Marie have done a gap analysis - They need to get the cells from Birmingham to start doing tests on transwells and the carousel - Rosh has started defrosting cells - They have also started looking at clever cell dish designs - Ken found one with RCF sandwiched in between that he will send over to them - Transwells are made for certain sizes: 12mm or 24mm - 12mm would make more sense for us - Requires at least 3 replicates - Marie to test this with the x-ray irradiator - Investigate if they get enough cells - Smaller dish also means more beam to use for instrumentation - Need a technical document on the transwells as well - Marie to send specifications of the transwells to Tony. - Send some to Birmingham so Tony (Emma) can test the transwells there as well. 3. Instrumentation - RCF - Updates on EBT4 delaminated - Asked but everyone been on holiday - The self-attempts were not working too well - SciFi - Calvin collecting the camera equipment to test from QMUL - Ken, Josie and Calvin to meet tomorrow to take this forward 5. Beamline Simulations - Fatima has joined us who works at ELI-NP - Going to help simulate ELI and Josie will try and reproduce her simulations - Josie waiting on Ewan and Robbie to return to understand her simulations further and get some more information - Josie has been talking to Hywel about the dipole designs 6. SCAPA - Updates 7. Review Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar 8. DoNM - 17/09/26 9. AoB - WELCOME FATIMA!!! === Summary of actions required === In-Beam Diagnostics - **Unassigned**: Acquire a glass sheet - **Unassigned**: Get a new RCF batch and calibrate - **TP**: Testing delaminating EBT3 - **PH**: Obtain transparent fibre or an alternative method for light transport - **PH**: Obtain a lens for hollow fibre tests - **CD**: Simulate energy deposited in transport fibres - **KL, CD, JMcG, PH**: Design a system to make full scifi prototypes - **KL, CD, JMcG, PH**: Make a full scifi prototype - **KL, CD**: Send clear fibre to SCAPA before 16th August (Contact Ewan or Colin since Robbie is USA) - **KL, CD, JMcG, PH**: Discuss visit to Birmingham to test SciFi - **RW, CD**: Study the correlation between laser diagnostics and mean dose - **Unassigned**: Investigate how a phosphor sheet could be incorporated into the design of the cell dish - **CW**: Investigate the feasibility of ICTs for our beamline - **MH**: Investigate LET detector - **CW**: Ask Narender about air fluorescence implementation - **JMcG**: Investigate using IF as a dosimeter - **GS, RH**: Investigate fast electron diagnostic - **TP**: Investigating commerical delaminated EBT4 Bio Next Steps - **EM, CD, JMcG**: Write up technical summary of Phase 2 - **EM**: Write up a biology plan for a 2 PMQ setup - **Unassigned**: Obtain an inverted microscope - **Unassigned**: Obtain multi-chamber haemocytometers (Might be able to use Birmingham's) - **Unassigned**: Obtain a thermostat for the incubator - **EM**: Send cells to Rosh and Marie - **EM, MB, RA**: Run controls to examine how long the cells can survive vertically - **JMcG**: Plan a test of vertical cell survival using Emma/Jason's HeLa cell line and Marie's cell line - **JMcG**: Produce a HeLa colony that can survive on Mylar - **CW, AM**: Show Alex around SCAPA - **MB, RA**: Send specifications of and transwells to Tony to test at Birmingham - **MB, RA**: Test transwells with x-rays - **KL**: Share paper with interesting cell dishes (RCF sandwich) Beamline Modelling - **Unassigned**: Investigate how to improve the beamline beyond CV=8% - **CD**: Examine energy spectra variation - **JMcG, ED**: Discuss spectra fits and SCAPA source divergence - **JMcG**: Talk to Darsebury to get more information on sensible dipole parameters Alternate Beamlines - **JMcG**: Simulate BELLA - **JMcG**: Simulate ELI - **CD**: Plannig doc for ELI ----