= LhARA radiobiology; meeting: 14May26; 14:00 GMT =

**ZOOM:** https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1 

Attended: PH, JMcG, MB, TP, CD, (KL)

== Meeting Notes == 

Plan for PoPLaR
1. Next SCAPA beamtime
 - Dates: July/August
 - Determine a baseline:
  - A baseline of repeating phase 2 cell irradiations was set

2. Aim for October
 - Paper
  - Aim for a physics publication with reference to the biology results
 - Add to Phase 3 plan:
  - Comet analysis provides DNA damage results and relatively easy to complete but requires more cell irradiations
   - Neutral and alkaline analyses seem the best to study single- and double-strand breaks
   - Also see the variation across the cell dish
  - Compare this and the clonogenics to the cyclotron irradiation
 - Questions to answer: 
  - Is the spatial variation acceptable?

3. Aim for Post-October
 - Desired work/papers
  - Include IF but it is unnecessary to do in Phase 3 due to the additional irradiations required
  - X-ray comparison: Feasible to be included in Phase 3
   - Would reproduce the same irradiation conditions (time out of incubator and vertical irradiation) as laser-driven protons
 - What do we need to know before we can plan more
  - Pre-proton electrons: 
   - Could explore using the dipoles to remove these
   - Have finance for the dipole but need stands so unlikely to be Phase 3
  - LET measurement
   - LET detector researched but unknown if it will be available for Phase 3

4. ELI
 - Almost certainly post-October and ran out of time to properly discuss how this can be included in the plan.

5. Review steps required
 - Plan on Google Doc: https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?usp=sharing
 - Steps on Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar
 - Updates:
  - Diagnostics:
   - RCF:
    - Tony is delaminating some RCF and will compare with the delaminated EBT3 Mark supplied. 
    - If we provide a batch we will use at SCAPA Tony can test this as well and do the calibration.
    - Suggestion that X-ray calibration removes the high-LET saturation effect.
    - Marie has offered to do the RCF calibration prior to travelling up
   - SciFi:
    - Peter has found a source for hollow fibre.
     - Cost: £300-400 for 15m, though Peter has money that can be put towards that.
     - Could do with a simulation to provide an order of magnitude estimate on the energy deposited on transport fibres
    - Tony has offered Birmingham to test the fibres before SCAPA
     - Make a package of everything we need and take to Birmingham 
     - Also, would be able to test using a fibre around the cell dish here
      - Keep this simple at the start and just use one fibre
  - Bio:
   - Could potentially borrow Marie's inverted microscope but depends on other lab users
   - Marie is keen to meet up with Jason and Emma to help organise a dry run or testing on the control cell survival

6. DoNM 
 - 21/05/26?

7. AoB
 - IPAC submitted

=== Summary of actions required ===

In-Beam Diagnostics
- **CD, TP**: Refine evaluations of LET in the cells with the RCF in front
 - **DONE**
- **CD**: Predict the cell dish dose from the RCF in front of it
 - **DONE**
- **PH, CD**: Initial measurements of sci-fi arrays
 - **DONE**
- **CD/KL**: Provide plan for Master's students who will count photons from UV source for sci-fi arrays
 - **DONE**
- **Unassigned**: Acquire a glass sheet
- **Unassigned**: Get a new RCF batch and calibrate
- **PH**: Investigate how to get the light out of the vacuum chamber
- **CD**: Simulate energy deposited in transport fibres
- **KL, CD, JMcG, PH**: Make a full scifi prototype
- **KL, CD, JMcG, PH**: Discuss visit to Birmingham to test SciFi
- **RW, CD**: Study correlation between laser diagnostics and mean dose
- **Unassigned**: Investigate how a phosphor sheet could be incorporated into the design of the cell dish

Bio Next Steps
- **CD**: Gather ideas for improving cell survival
 - **DONE**
- **EM, CD, JMcG**: Write up technical summary of Phase 2
- **EM**: Write up a biology plan for a 2 PMQ setup
- **Unassigned**: Obtain an inverted microscope
- **Unassigned**: Obtain multi-chamber haemocytometers (Might be able to use Birmingham's)
- **MB, JP, MB**: Meet up to plan cell survival dry run
- **EM*: Run controls to examine how long the cells can survive vertically

Beamline Modelling
- **CD**: Find LET for different energies in the cell dish
 - **DONE**
- **RW**: Examine energy spectra variation
- **RW, CD**: Source characterisation

Other Beamlines
- **EM**: Coordinate ELIMED bid
 - **DONE**
- **JMcG**: Simulate BELLA
- **JMcG**: Simulate ELI

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