Changes between Version 1 and Version 2 of Research/LhARA/RadiationBiology/BMthdlgy/ImprvCllSrvvl


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Timestamp:
Apr 16, 2026, 5:12:24 PM (7 weeks ago)
Author:
ccd24
Comment:

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  • Research/LhARA/RadiationBiology/BMthdlgy/ImprvCllSrvvl

    v1 v2  
    88 - Not irradiating RCF in the same carousel
    99  - A combination of these two would bring the irradiation time from roughly 16 mins to roughly 4 mins
    10 - Dealing with potential causes: Temperature, CO2, lack of media
     10- Dealing with potential causes: Temperature, CO2, lack of media, weak cell line
    1111 - Heat the carousel in which the cells are placed
    1212 - Keep the carousel rotating so that the cells are consistently wet
    1313 - Change the cell dish lid so that more media can be added through a second hole
    1414  - Would also help regulate temperature
    15 - Change the cell line from HeLa to the super-resistant ones used in Marie's lab
    16  - Requires testing the cells at Birmingham and repeating the experiments
    17  - Not certain it will work
     15 - Change the cell line from HeLa to the super-resistant ones used in Marie's lab
     16  - Requires testing the cells at Birmingham and repeating the experiments
     17  - Not certain it will work
    1818- Equipment factors
    1919 - Place a thermostat in the incubator to check that it is not too warm