Changes between Version 1 and Version 2 of Research/LhARA/RadiationBiology/BMthdlgy/ImprvCllSrvvl
- Timestamp:
- Apr 16, 2026, 5:12:24 PM (7 weeks ago)
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Research/LhARA/RadiationBiology/BMthdlgy/ImprvCllSrvvl
v1 v2 8 8 - Not irradiating RCF in the same carousel 9 9 - A combination of these two would bring the irradiation time from roughly 16 mins to roughly 4 mins 10 - Dealing with potential causes: Temperature, CO2, lack of media 10 - Dealing with potential causes: Temperature, CO2, lack of media, weak cell line 11 11 - Heat the carousel in which the cells are placed 12 12 - Keep the carousel rotating so that the cells are consistently wet 13 13 - Change the cell dish lid so that more media can be added through a second hole 14 14 - Would also help regulate temperature 15 - Change the cell line from HeLa to the super-resistant ones used in Marie's lab16 - Requires testing the cells at Birmingham and repeating the experiments17 - Not certain it will work15 - Change the cell line from HeLa to the super-resistant ones used in Marie's lab 16 - Requires testing the cells at Birmingham and repeating the experiments 17 - Not certain it will work 18 18 - Equipment factors 19 19 - Place a thermostat in the incubator to check that it is not too warm
