LhARA radiobiology; meeting: 14Jul25; 14:00 BST

Present: CW, RW, CP, EM, JMcG, AFR, CD, KL, DO

Notes

1. Minutes and actions:

• JP: Talk to Fisher and get a plan of action for the order and delivery of consumables to Strathclyde
  • STANDS
• CD/AFR: Complete verification of RCF stack
  • STANDS

2. Strathclyde Updates: RW, CW
   

• Laser damage and beamtime:
  • Damage also includes a vacuum pipe that needs fixing, defines a new schedule
  • New Schedule:
    • Week 1: Get back to where we were a month ago with correct laser delivery to the target.
    • Week 2: Re-establish and troubleshoot proton generation.
    • Week 3: Work with the quads and some diagnostic testing in the PoPLaR configuration.
      • Opportunity for a full bio walkthrough.
      • Double check the autoclave waste
      • Highly dependent on week 1 and week 2

• Bio-safety cabinets:
  • Recalibration still required and unsure of schedule

• RCF stack fiduciary:
  • Smaller stack proving to be tricky
  • Larger stack can be used as a viable back-up
  • Ken may be able to encourage CLF to help with smaller stack
  • Aligned with a cross-hair on carousel and a laser along the beam axis

6. Bioprep: EM/MH
   
   • IF needs staining from the last experiments (14 vs 28 MeV)
   • Issue with Mylar remains - colony survival too variable to be reliable
   • Tested post-plating FaDu cells from Mylar following 28 MeV protons (0-4Gy) with reliable and consistent colony formation
   • To do - Rerun cyclotron test experiments comparing 14 and 28 MeV with post-plating method for both HeLa and FaDu - date TBD
   • To do - stain, image and analyse IF on Mylar

7. DoNM
   • 22Jul25 14:00 BST

8. AoB
   
   • Present work to LhARA fortnightly tomorrow
     • CD: Updated SCAPA schedule
     • EM: Updates from latest experiment
     • CD: LMU work

Summary of actions

EM: Comparison of 14 and 28 MeV with post-plating method. Analyse IF on Mylar CW: Bio-cabinets need recalibration

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