Changes between Version 4 and Version 5 of Research/LhARA/RadiationBiology/Meetings/2025-11-27
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- Nov 27, 2025, 5:04:56 PM (12 days ago)
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Research/LhARA/RadiationBiology/Meetings/2025-11-27
v4 v5 3 3 **ZOOM:** https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1 4 4 5 Attended: EM, JMcG, CW, DA, AFr, TP, AG, MB, MH, JP, RW, CD 5 6 6 == Agenda==7 == Meeting Notes == 7 8 8 9 1. Minutes and actions: … … 10 11 2. Laser: RW \\ 11 12 - RW: Explanation of the laser problem and the fix 13 - That problem seems permanently fixed. 12 14 13 15 3. RCF: AFR, DA, CD \\ 14 16 - Calibration 15 17 - Rotation Issue 18 - Rotating the films in the scanner can cause a ~30% change to the mean dose in a particular case. More generally causes a substantial difference. 19 - Can combat by rotating the films each time we scan and always selecting the orientation that gives the highest mean pixel count 16 20 - Calculating Dose per Shot, Variation and Dose delivered to Cells 21 - From RCF, average dose per shot of 0.61±0.25Gy (More data to be analysed) 22 - Hard to spot a variation day to day 23 - Variation in one showed a change after 8 films had been irradiated. Need to analyse the shots to get a better understanding of what this is 24 - Robbie's suggestion is a defocussing laser spot effect 17 25 - Flatness and Uniformity of Dose 18 - (Comparison with Sim) 26 - Average CV: 15.1±2.5% 27 - Average Flatness in X: 26.4±5.5% 28 - Average Flatness in Y: 29.6±4.7% 29 - Beam is too non-uniform still 30 - Sim comparison 31 - CV: 17% 32 - Moving the Copper closer would bring CV down to 9% and the mean dose would also fall by 45% 19 33 20 34 4. Bio: EM, JP \\ 21 35 - EM: Summary of Experiment 36 Dry Run 37 - Seeding density test 38 FaDu gave a plating efficiency of 40?% 22 39 - Marie Boyd update 23 40 - Comet analysis 41 Alive dead stain can be done after a couple of hours? 42 That depends on the dose received to how to wait 43 - Collect pellets and do a western blot test for a rough idea 44 - Could not tell between a 2 and 4Gy 45 H2X 46 -There is a way to select cells from all the same area. Though there is the issue that there are not enough cells then. 47 48 - IF might give an idea of hotspots 49 - x-ray irradiate with a known dose to do a comparison 50 - Marie will do a comet assay 24 51 - Plan for staining and delivery back to Birmingham 52 53 - Move things to Marie's lab 54 - Can also do some control irradiations at Marie's as well 55 56 57 - From the colonies, no growth in the controls either 58 - Microscope is not great, so Marie can take their one to SCAPA 59 - Could be the microscope that means cannot see any cells 60 - Marie can do the staining check on Friday but will do on Monday 61 - The cells were dried out by 50%, incubator felt very hot and humid. Put a thermometer in it to check 25 62 26 63 5. Future: \\ … … 28 65 - Clear Communication of Requirements 29 66 - RCF to Dose Procedure 30 - Uniformity? 67 - Alfredo and Diaza are piecing together a protocol document to standardise how we complete this procedure 68 - The rotation issue is taken into account 69 - Diaza in favour of scanning at lower resolution to speed it up 70 - Uniformity not good enough 71 - Place the Copper scatterer closer 72 - Results need to analysed 31 73 - Shot-to-shot variation 32 - Discussion of Solutions 33 - Short-term (before the next SCAPA visit) and long-term 74 - Include a piece of RCF in front of the cells (Ideally delaminated but not possible to calibrate before the next visit) 75 - Need to sim what the LET would be for our energy range into the cells with the RCF in place 76 - Longer-term investigate an on-beam diagnostic 77 - Another option is to find a correlation between the RCF mean dose and one of the laser diagnostics that the team already gathers 78 34 79 - Discussion of when to use the rollover days 35 - Need to wait until after the biology results have been analysed 36 - Book a longer discussion to analyse all the results and define a plan for the future. 80 - Plan to use Wednesday, Thursday and Friday next week 81 - If good, then use the 3 days to do more cell irradiations 82 - Emma will send the dishes up tomorrow 83 - If not work on more beam diagnostics and improving it to complete cell irradiations, tackling either uniformity or shot-to-shot variation 84 37 85 - IPAC abstract submission? 86 - No takers and a belief that we should wait until we have a slam dunk result 87 - Note: LhARA will have a general submission that will mention PoPLaR 88 - Book a longer discussion to analyse all the results and define a plan for the future 89 - Not discussed 38 90 39 91 6. DoNM … … 41 93 42 94 7. AoB 43 - Emma Viva 95 - Congratulations Dr Melia!!! 96 97 98 99 44 100 ----
