| 35 | | - EM: Summary of Experiment |
| 36 | | Dry Run |
| 37 | | - Seeding density test |
| 38 | | FaDu gave a plating efficiency of 40?% |
| 39 | | - Marie Boyd update |
| 40 | | - Comet analysis |
| 41 | | Alive dead stain can be done after a couple of hours? |
| 42 | | That depends on the dose received to how to wait |
| 43 | | - Collect pellets and do a western blot test for a rough idea |
| 44 | | - Could not tell between a 2 and 4Gy |
| 45 | | H2X |
| 46 | | -There is a way to select cells from all the same area. Though there is the issue that there are not enough cells then. |
| | 36 | - Dry run seeding density test has worked: [raw-attachment: Slides] |
| | 37 | - Marie/Rosh are going to check colonies on Friday/Monday and make executive decision on staining days |
| | 38 | - Test the temperature of the incubator, as it seems too warm |
| | 39 | - Incubator water dried out, Rosh has refilled (some indication the plates were drying out ~50% - check how much as only 3ml was added. Could top up with fresh media) |
| 53 | | - Move things to Marie's lab |
| 54 | | - Can also do some control irradiations at Marie's as well |
| 55 | | |
| 56 | | |
| 57 | | - From the colonies, no growth in the controls either |
| 58 | | - Microscope is not great, so Marie can take their one to SCAPA |
| 59 | | - Could be the microscope that means cannot see any cells |
| 60 | | - Marie can do the staining check on Friday but will do on Monday |
| 61 | | - The cells were dried out by 50%, incubator felt very hot and humid. Put a thermometer in it to check |
| | 43 | - Wait for staining (& analysis?) of current clonogenics before irradiating more cells |
| | 44 | - If no survival in the control plates is seen, procedure needs evaluating as dry run was ok |
| | 45 | - Issues for running cells next week: Cells/Mylar dishes/lids need to be taken from Birmingham - discussions regarding specific ideal days and people availability are needed |
| | 46 | - Next run - do comparable experiments with Marie/Rosh and the X-ray source and possibly add RCF to base of the cell dish. |
| | 47 | - Discussion of faster cell analysis techniques such as western blot |
| | 48 | - Use IF to get an idea of hotspots in the cell dish? |
| | 83 | - [raw-attachment:Emma_Congrats.pptx pic] |
| | 84 | |
| | 85 | === Summary of actions required === |
| | 86 | == Short-Term == |
| | 87 | - **MB**: Evaluate the cells |
| | 88 | - **EM**: Send the cell dishes to SCAPA if required and decide on the shot plan |
| | 89 | - **CD, RW, EM, CW ...**: Plan what to run if cells not required. Lay out a clear aim for the rollover days. |
| | 90 | - **CD, TP**: Evaluate LET in the cells with the RCF in front |
| | 91 | - **CD, AFr**: Complete analysis of RCF with Error Calculation |
| | 92 | - **AFr, DA**: Write out RCF procedure document |
| | 93 | - **TP**: Test orientation of calibration RCF in scanner at Birmingham |
| | 94 | - **CD**: Send shot results to Robbie to help find the cause of variation after 8 films irradiated |
| | 95 | |
| | 96 | == Long-Term == |
| | 97 | - **ALL**: Organise a longer meeting discussion post rollover days |
| | 98 | - **RW, CD**: Study correlation between laser diagnostics and mean dose |
| | 99 | - **EM, JMcG**: Write up summary of PoPLaR Phase 2 |
| | 100 | - **KL**: Investigate other on-beam diagnostics |
| | 101 | - **CD, JMcG**: Investigate how to achieve uniformity without scatterer in place |
| | 102 | |
