Changes between Version 3 and Version 4 of Research/LhARA/RadiationBiology/Meetings/2026-02-05

Timestamp:

Feb 5, 2026, 3:22:20 PM (4 months ago)

Author:

ccd24

Comment:

--

Research/LhARA/RadiationBiology/Meetings/2026-02-05

@@ -3 +3 @@
 **ZOOM:** https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1 
 
-== Agenda == 
+Attended: KL, RW, CW, RA, PH, MB, AFr, MH, EM, CD
+
+== Meeting Notes == 
 
 1. Minutes and actions completed:
@@ -14 +16 @@
 - Date: DECIDED 24th Feb at Birmingham
 - Discussion on location
-- Draft Agenda
+ - Confirmed Birmingham. Jason and Emma to book a room for max 20 people.
+ - Invite LMU to join online
+ - Invite Liverpool to share their work on air scintillation
+ - Invite Chris Armstrong to join for instrumentation section
+- Draft Agenda: https://indico.stfc.ac.uk/event/1834/ 
+ - Make clear to include timetables for SCAPA and ELIMED (and LMU)
+ - Make clear a section on improved survival rates of cells
 
 3. SCAPA RW,ED:
 - General Update
+ - Laser fixed
+ - Running a DSTL till mid-march, going to be around April before access again
+ - Josie asked for Dipoles from IoM charity. Waiting outcome
 
 4. Bio EM,JP,MB:
 - Actions Checklist
  - **MB/EM**: Send and evaluate the cells
+  - Delivered
  - **EM, JMcG**: Write up summary of PoPLaR Phase 2, including technical summary of cell irradiation procedure
  - **EM**: Share the counting software she uses with Marie
+  -**Done**
+  - Oxford Optronix, Gelcount
  - **MB**: Comet analysis
 - General Updates
+ - Cells have been delivered to Birmingham
+  - Looks like nothing on HeLa. FaDu looks more promising
+  - Caution required due to dose variation
+ - Before next run inform Marie the dates so she can book out the incubator to be used
+ - Ideas to improve cell survival rates
+  - Swap out HeLa with Glioma cell line (EBW). More robust.
+   - Would need to send some to Birmingham
+   - Emma to email Rosh about when to take them out the freezer
+  - Dry runs indicate that if the cells are only out for 20/25mins then good survival
+   - Remove the RCF after irradiation reduces time drastically
+  - Cells could be too cold
+   - Heating the whole holder could provide enough heat to keep the cells warm for longer
+  - Cells could be hypoxic
+   - Pre-gas the cells?
+  - Keep the holder rotating so that the cells stay wet during irradiation
 
 5. Beamline Diagnostic, KL, TP, CD, PH:
 - Actions Checklist
  - **EM/ED/LJ**: Send calibration films to SCAPA and scan them in both orientations
+  - Delivered
  - **AFr, DA**: Write out RCF procedure document
   - **DONE**
@@ -36 +66 @@
   - **DONE**
 - Protons per Fibre - CD
+ - [raw-attachment: Slides]
+ - Estimating that the beam delivers 0.2mJ per shot per fibre, which works to 200W
+ - Oversaturation problem?
 - Overview of RCF document
+ - [raw-attachment: Doc]
 - General Updates
+ - CD, RW and AFr to discuss the best way to scan RCF
 
 6. DoNM 
@@ -43 +78 @@
 
 7. AoB 
+- Prepare a summary for LhARA fortnightly on the day of the meeting
 
 === Summary of actions required ===
 
 Phase 2 Analysis
-- **EM, MB**: Organise transport of cells to Birmingham for evaluation
 - **EM**: Evaluate the cell results
 - **CD, AFr**: Complete analysis of RCF with errors
 - **EM/ED/LJ**: Send calibration films to SCAPA and scan them in both orientations
+ - **CD, RW and AFr**: Explain scanning procedure
 - **EM, JMcG**: Write up summary of PoPLaR Phase 2, including technical summary of cell irradiation procedure
-- **EM**: Share the counting software she uses with Marie
 - **MB**: Comet analysis
 
@@ -61 +96 @@
 
 Improve Bio results
-- **EM, JP**: Discuss cell line to use next time?
+- **EM**: Inform Roshine when to defrost Glioma cell line
 - **Unassigned**: Obtain an inverted microscope