Changes between Version 5 and Version 6 of Research/LhARA/RadiationBiology/Meetings/2026-15-14

Timestamp:

May 14, 2026, 5:12:31 PM (7 days ago)

Author:

ccd24

Comment:

--

Research/LhARA/RadiationBiology/Meetings/2026-15-14

@@ -3 +3 @@
 **ZOOM:** https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1 
 
-== Meeting Agenda == 
+Attended: PH, JMcG, MB, TP, CD, (KL)
+
+== Meeting Notes == 
 
 Plan for PoPLaR
 1. Next SCAPA beamtime
  - Dates: July/August
- - Determine a baseline/default
-  - Steps required for a better repeat of the cell irradiations done previously
-   - Google doc summary: https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?usp=sharing
-   - Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar
+ - Determine a baseline:
+  - A baseline of repeating phase 2 cell irradiations was set
 
 2. Aim for October
- - Paper(s)?
- - What changes are required to that baseline plan for SCAPA to achieve that by October
+ - Paper
+  - Aim for a physics publication with reference to the biology results
+ - Add to Phase 3 plan:
+  - Comet analysis provides DNA damage results and relatively easy to complete but requires more cell irradiations
+   - Neutral and alkaline analyses seem the best to study single- and double-strand breaks
+   - Also see the variation across the cell dish
+  - Compare this and the clonogenics to the cyclotron irradiation
+ - Questions to answer: 
+  - Is the spatial variation acceptable?
 
 3. Aim for Post-October
  - Desired work/papers
+  - Include IF but it is unnecessary to do in Phase 3 due to the additional irradiations required
+  - X-ray comparison: Feasible to be included in Phase 3
+   - Would reproduce the same irradiation conditions (time out of incubator and vertical irradiation) as laser-driven protons
  - What do we need to know before we can plan more
- - What can we do pre-October to help that
+  - Pre-proton electrons: 
+   - Could explore using the dipoles to remove these
+   - Have finance for the dipole but need stands so unlikely to be Phase 3
+  - LET measurement
+   - LET detector researched but unknown if it will be available for Phase 3
 
 4. ELI
- - Dates?
- - How does this fit into the plan
- - What steps need to be added to be prepared for this beamtime
+ - Almost certainly post-October and ran out of time to properly discuss how this can be included in the plan.
 
 5. Review steps required
- - Share Trello
+ - Plan on Google Doc:https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?usp=sharing
+ - Steps on Trello:https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar
+ - Updates:
+  - Diagnostics:
+   - RCF:
+    - Tony is delaminating some RCF and will compare with the delaminated EBT3 Mark supplied. 
+    - If we provide a batch we will use at SCAPA Tony can test this as well and do the calibration.
+    - Suggestion that X-ray calibration removes the high-LET saturation effect.
+    - Marie has offered to do the RCF calibration prior to travelling up
+   - SciFi:
+    - Peter has found a source for hollow fibre.
+     - Cost: £300-400 for 15m, though Peter has money that can be put towards that.
+     - Could do with a simulation to provide an order of magnitude estimate on the energy deposited on transport fibres
+    - Tony has offered Birmingham to test the fibres before SCAPA
+     - Make a package of everything we need and take to Birmingham 
+     - Also, would be able to test using a fibre around the cell dish here
+      - Keep this simple at the start and just use one fibre
+  - Bio:
+   - Could potentially borrow Marie's inverted microscope but depends on other lab users
+   - Marie is keen to meet up with Jason and Emma to help organise a dry run or testing on the control cell survival
 
 6. DoNM 
- - 28/05/26
+ - 21/05/26?
 
 7. AoB
@@ -50 +81 @@
 - **Unassigned**: Get a new RCF batch and calibrate
 - **PH**: Investigate how to get the light out of the vacuum chamber
+- **CD**: Simulate energy deposited in transport fibres
 - **KL, CD, JMcG, PH**: Make a full scifi prototype
+- **KL, CD, JMcG, PH**: Discuss visit to Birmingham to test SciFi
 - **RW, CD**: Study correlation between laser diagnostics and mean dose
 - **Unassigned**: Investigate how a phosphor sheet could be incorporated into the design of the cell dish
@@ -61 +94 @@
 - **Unassigned**: Obtain an inverted microscope
 - **Unassigned**: Obtain multi-chamber haemocytometers (Might be able to use Birmingham's)
+- **MB, JP, MB**: Meet up to plan cell survival dry run
 - **EM*: Run controls to examine how long the cells can survive vertically