Changes between Version 1 and Version 2 of Research/LhARA/RadiationBiology/Meetings/2026-08-20


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Timestamp:
Aug 20, 2026, 4:39:03 PM (2 weeks ago)
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ccd24
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  • Research/LhARA/RadiationBiology/Meetings/2026-08-20

    v1 v2  
    77- Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar
    88
    9 == Meeting Agenda ==
     9== Meeting Notes ==
    1010
    11111. Beamline Simulation
    12  - Josie: Characterisation of ELI and SCAPA sources
     12 - Josie: Characterisation of ELI and SCAPA sources [raw-attachment: Slides]
     13  - Using the LLO simulation and changing the electron temperature in the model to match the energy spectra for the ELI and SCAPA beamlines
     14  - SCAPA fitted with 5.5MeV electron temperature
     15  - ELI fitted with 0.25MeV electron temperature
     16  - Will chat with Ewan to discuss further
     17  - Need information on the source divergence
    1318 - Josie: Simulations of a dipole at SCAPA
     19  - Have funding for dipoles
     20  - First introduction of dipoles indicates a 90% beam loss
     21  - Need a more accurate measurement of the space to work in
     22  - Can test at Birmingham
     23  - Josie to talk to Darsebury to get more information on sensible dipole parameters
     24  - Next beamtime will probably not include this dipole arrangement
    1425
    15 2. Instrumentation
     263. BioPrep
     27 - Alex's plan: [raw-attachment: Slides]
     28  - Use transwell with 10um membrane
     29  - Use absorbent paper on the side of transwell with media
     30   - Don't need cells to be in media
     31   - Concern that as the cells/paper dries the thcikness of media will change and the cell will receive a different dose?
     32  - No cell lid and no media means we can place RCF behind the cells
     33   - With the cells being vertical little concern over contamination
     34   - Could cover all but one of the holes on the carousel with a Perspex cover
     35    - This will keep the moisture in the dish
     36    - Uncover just to irradiate and then rotate back under
     37    - This also works for a vertical beam so sterilisation is not an issue
     38     - Maybe add a second plastic screen above the beamline just in case
     39    - Can always use antibacterial media
     40  - Have 6, 12, and 24 well plates so we can cluster the cells that all see the same dose ie reduces the spatial variation.
     41  - Also could flash-freeze the media and do analysis on that (bystander)
     42  - Whether electrical impedance to check the cell layer is a monolayer and other techniques
     43 - Need to dry run and understand cell survival
     44  - Colin to take Alex on a tour of SCAPA (Aiming for next week)
     45 - Need an understanding of the publishable minimum
     46  - Roshine and Marie to draft a plan and to share with Emma and Jason (and Josie)
     47  - Incubator downstairs in SCAPA?
     48   - Consider the CO2
     49  - Minimum repeats is 3
     50  - Minimum cell lines is 2
     51  - Which cell lines
     52   - Emma and Jason prefer their HeLa and FaDu since already done Birmingham runs with them
     53   - Think about using the hardier cell lines from Glasgow as well
     54
     553. Instrumentation
    1656 - Fast Electrons
    17   - Richard H.: Findings since last meeting
     57  - Giuseppe and Richard H. looking at this and need to find the apparatus with the right parameters for our system
     58  - Ken to visit on October
    1859 - RCF
    19   - Update from NPL
    2060  - Mark has found some EBT4 delaminated: https://www.ashland.com/industries/medical/radiotherapy-films/ebt4
     61  - Tony to inquire to see if he can get hold of some
     62  - Alex also suggests casting the RCF into a thinner sheet by using an organic solvent and casting it onto a silicon sheet.
    2163 - Air Scintillation
    2264  - Colin/Narendar: Planning to meet to discuss the feasibility of the implementation at SCAPA and the timeframe
    2365
    24 3. BioPrep
    25  - Notes from meeting with Alexander Mullen
    26   - Vertical beam needed?
    27 
    28664. SCAPA
    29  - Updates
     67 - Updates: None (On experiment)
    3068
    31695. Review Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar
    … …  
    5593- **CW**: Ask Narender about air fluorescence implementation
    5694- **JMcG**: Investigate using IF as a dosimeter
     95- **GS, RH**: Investigate fast electron diagnostic
     96- **TP**: Investigating commerical delaminated EBT4
    5797
    5898
    … …  
    66106- **JMcG**: Plan a test of vertical cell survival using Emma/Jason's HeLa cell line and Marie's cell line
    67107- **JMcG**: Produce a HeLa colony that can survive on Mylar
     108- **CW, AM**: Show Alex around SCAPA
    68109
    69110Beamline Modelling
    70111- **Unassigned**: Investigate how to improve the beamline beyond CV=8%
    71112- **CD**: Examine energy spectra variation
    72 - **RW, CD**: Source characterisation
     113- **JMcG, ED**: Discuss spectra fits and SCAPA source divergence
     114- **JMcG**: Talk to Darsebury to get more information on sensible dipole parameters
    73115
    74116Alternate Beamlines