LhARA Radiobiology Meeting: 03Sep26; 14:00 BST

ZOOM: ​https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1

• Planning Document: ​https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?tab=t.0

• Trello: ​https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar

Meeting Agenda

1. Alternate Beamlines
   • ELI proposal feedback
     • Got rejected for several reasons but most are things we can deal with and the evaluators were generally positive and encouraged resubmission
     • Next proposals for ELI have a deadline in October
       • Beamtime next year
     • Ken checking whether ELIMED is part of that
     • Calvin to make a planning document so we can list the challenges and fill out how we will deal with them
       • Discuss with ELI instrumentation team to make acceptance as likely as possible
     • Discussion on beam energy
       • Marie and Rosh have done a gap analysis and found one looking at <10MeV protons
       • Fear that focussing on lower energy will not appeal to ELI since they have the technology to reach 60MeV
       • ELI also appears to have lower beam quality as you go to lower energy
       • Jason has comparison at Clatterbridge for 60MeV and 28MeV
       • Could suggest higher energies but also no clear indication that lower energy was a reason we got rejected since we did not mention beam energy in the original submission

2. BioPrep
   • Ken and Josie did a review
     • Ken showed a figure showing cell death and very little difference between laser-driven and conventional but also multi-pulse and single shots
       • Aim should be to improve on this
   • Rosh and Marie have done a gap analysis
     • They need to get the cells from Birmingham to start doing tests on transwells and the carousel
     • Rosh has started defrosting cells
     • They have also started looking at clever cell dish designs
     • Ken found one with RCF sandwiched in between that he will send over to them
   • Transwells are made for certain sizes: 12mm or 24mm
     • 12mm would make more sense for us
     • Requires at least 3 replicates
     • Marie to test this with the x-ray irradiator
     • Investigate if they get enough cells
     • Smaller dish also means more beam to use for instrumentation
     • Need a technical document on the transwells as well
     • Marie to send specifications of the transwells to Tony.
     • Send some to Birmingham so Tony (Emma) can test the transwells there as well.

3. Instrumentation
   • RCF
     • Updates on EBT4 delaminated
     • Asked but everyone been on holiday
   • The self-attempts were not working too well
   • SciFi
     • Calvin collecting the camera equipment to test from QMUL
     • Ken, Josie and Calvin to meet tomorrow to take this forward

5. Beamline Simulations
   • Fatima has joined us who works at ELI-NP
     • Going to help simulate ELI and Josie will try and reproduce her simulations
   • Josie waiting on Ewan and Robbie to return to understand her simulations further and get some more information
   • Josie has been talking to Hywel about the dipole designs

6. SCAPA
   • Updates

7. Review Trello: ​https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar

8. DoNM
   • 17/09/26

9. AoB

• WELCOME FATIMA!!!

Summary of actions required

In-Beam Diagnostics

• Unassigned: Acquire a glass sheet
• Unassigned: Get a new RCF batch and calibrate
• TP: Testing delaminating EBT3
• PH: Obtain transparent fibre or an alternative method for light transport
• PH: Obtain a lens for hollow fibre tests
• CD: Simulate energy deposited in transport fibres
• KL, CD, JMcG, PH: Design a system to make full scifi prototypes
• KL, CD, JMcG, PH: Make a full scifi prototype
• KL, CD: Send clear fibre to SCAPA before 16th August (Contact Ewan or Colin since Robbie is USA)
• KL, CD, JMcG, PH: Discuss visit to Birmingham to test SciFi
• RW, CD: Study the correlation between laser diagnostics and mean dose
• Unassigned: Investigate how a phosphor sheet could be incorporated into the design of the cell dish
• CW: Investigate the feasibility of ICTs for our beamline
• MH: Investigate LET detector
• CW: Ask Narender about air fluorescence implementation
• JMcG: Investigate using IF as a dosimeter
• GS, RH: Investigate fast electron diagnostic
• TP: Investigating commerical delaminated EBT4

Bio Next Steps

• EM, CD, JMcG: Write up technical summary of Phase 2
• EM: Write up a biology plan for a 2 PMQ setup
• Unassigned: Obtain an inverted microscope
• Unassigned: Obtain multi-chamber haemocytometers (Might be able to use Birmingham's)
• Unassigned: Obtain a thermostat for the incubator
• EM: Send cells to Rosh and Marie
• EM, MB, RA: Run controls to examine how long the cells can survive vertically
• JMcG: Plan a test of vertical cell survival using Emma/Jason's HeLa cell line and Marie's cell line
• JMcG: Produce a HeLa colony that can survive on Mylar
• CW, AM: Show Alex around SCAPA
• MB, RA: Send specifications of and transwells to Tony to test at Birmingham
• MB, RA: Test transwells with x-rays
• KL: Share paper with interesting cell dishes (RCF sandwich)

Beamline Modelling

• Unassigned: Investigate how to improve the beamline beyond CV=8%
• CD: Examine energy spectra variation
• JMcG, ED: Discuss spectra fits and SCAPA source divergence
• JMcG: Talk to Darsebury to get more information on sensible dipole parameters

Alternate Beamlines

• JMcG: Simulate BELLA
• JMcG: Simulate ELI
• CD: Plannig doc for ELI

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